207
Views
6
CrossRef citations to date
0
Altmetric
SENSORS

Electrogravimetric Analysis by Quartz-Crystal Microbalance on the Consumption of the Neurotransmitter Acetylcholine by Acetylcholinesterase

, , , , &
Pages 258-265 | Received 08 May 2012, Accepted 09 Jul 2012, Published online: 02 Jan 2013
 

Abstract

Electrogravimetric analysis was performed on the consumption of the neurotransmitter Acetylcholine (ACh) by Acetylcholinesterase (AChE) in situ and in real time. Michaelis-Menten assumption was achieved by using an enzyme micro-reactor in which the total enzyme was anchored in a quartz crystal microbalance chip (QCM-chip) with a strategically engineered self-assembled monolayer (SAM) of alkanethiols, which can prevent diffusion-controlled or spatially restricted kinetics. The real-time frequency changes indicated the rate of the products formation from enzymatic reaction. The QCM-chip was tested showing that it could demonstrate AChE inhibition by physostigmine.

Acknowledgments

The authors acknowledge the São Paulo State Research Funding Agency (FAPESP), the Brazilian National Research Council (CNPq), as well as the BIOTA program (FAPESP research center) and their researchers for their useful discussions. L. M. Gonçalves (SFRH/BD/76544/2011) wishes to acknowledge Portuguese Fundação para a Ciência e a Tecnologia (FCT) for his post-doctoral scholarship.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.