Combined Application of Ionic Liquid and Hybrid Poly (Ionic Liquid)-Bonded Silica: An Alternative Method for Extraction, Separation and Determination of Flavonoids from Plants

Abstract

Hybrid poly (ionic liquid)-bonded silica was combined with an ionic liquid solution for the extraction, separation, and determination of flavonoids from natural plants by using a multi-phase dispersive extraction (MPDE) method. The hybrid material was synthesized using a facile method. A suitable sorbent was identified based on the adsorption behaviors of flavonoids on different poly (ionic liquid)-bonded silicas. In contrast to traditional matrix solid-phase dispersion (MSPD) method, the target analytes were first extracted by three-phase (sample-solvent-sorbent) dispersive extraction with ionic liquid (1-oxyl-3-methylimidazolium bromide) solutions as the solvent, and then cleaned up after removal from the sample matrix, called MPDE. This process combines the advantages of ionic liquids, ionic liquid-based sorbent, and MPDE. The recovery rates were achieved by MPDE of the flavonoids from Chamaecyparis obtusa: 76.4% for myricetin and 90.3% for amentoflavone. The proposed method may be used to extract and separate other flavonoids or even polyphenolic compounds from complex samples.

Keywords:

• Chamaecyparis obtusa
• Determination
• Flavonoid
• Ionic liquid
• Multi-phase dispersive extraction
• Separation

Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (2012-0005250).

Notes

^a %C/%Cl = (12*3)/35.5; %Cl = 4.79.

^b %N/%Cl = (14*2)/35.5; %Cl = 6.06.