117
Views
7
CrossRef citations to date
0
Altmetric
IMMUNOASSAY

Biotin-Streptavidin-enhanced Enzyme-Linked Immunosorbent Assay for the Determination of Parathion-Methyl in Vegetables

, , , , &
Pages 1084-1096 | Received 23 Oct 2012, Accepted 09 Nov 2012, Published online: 10 Apr 2013
 

Abstract

The development of rapid, simple, and sensitive analytical methods for food contaminants is a topic of considerable interest. Polyclonal antibody against parathion-methyl (PM) was raised and used to develop a biotin-streptavidin indirect competitive enzyme-linked immunosorbent assay (BS-icELISA) with improved sensitivity for the determination of PM in vegetable. At the optimum conditions, the IC50 and limit of detection of BS-icELISA for PM were found to be 1.2 µg/L and 0.2 µg/L, respectively, which is 6-fold more sensitive than the traditional icELISA. This method was applied to determine PM residue in three vegetable samples with a simple and effective extraction procedure, good recoveries (in the range of 88.2–108.7% and with coefficients of variation below 15%) and accuracy (correlation coefficient of 0.9576 with GC-MS) were obtained. Our results indicated that the biotin-streptavidin system is useful in improving the sensitivity of ELISA and could be used for routine monitoring of pesticide at trace level.

Notes

a Natural antibody for icELISA and biotinylated antibody for BS-icELISA.

b IgG-HRP for icELISA and SA-HRP for BS-icELISA.

a Percentage of CR was calculated according to the following equation: CR(%) = [IC50 (PM, mmol/L)/IC50 (analogs, mmol/L)] × 100.

a The concentration spiked was lower than the limit of detection and undetectable.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.