Determination of Terpenoids in Chamaecyparis obtusa Leaves by Headspace Single-Drop Microextraction with Gas Chromatography Detection

Abstract

Headspace single-drop microextraction (HS-SDME) was used to extract five terpenoids (α-terpinene, γ-terpinene, linalool, α-terpineol, and terpinyl acetate) from powdered Chamaecyparis obtusa leaves. The terpenoids were determined by gas chromatography with flame ionization detection. Under the optimized conditions, oleyl alcohol efficiently extracted the analytes from the leaves within 20 min at 100°C. The results showed that the amounts of α-terpinene, γ-terpinene, linalool, α-terpineol, and terpinyl acetate extracted from the samples were 2.19, 3.68, 0.11, 0.87, and 22.35 µg/g, respectively. Compared to microextraction of the compounds from the sample solution, the new technique had fewer steps. The method also had similar extraction properties as heat reflux extraction, but required a smaller sample volume and less time.

Keywords:

• Chamaecyparis obtusa
• Gas chromatography
• Headspace single-drop microextraction
• Optimization
• Terpenoids

Acknowledgments

This study was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science, and Technology (No. 2011-0010673).