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LIQUID CHROMATOGRAPHY

Determination of Arbutin, Niacinamide, and Adenosine in Functional Cosmetic Products by High-Performance Liquid Chromatography

, , , , , & show all
Pages 1650-1660 | Received 30 Sep 2013, Accepted 05 Jan 2014, Published online: 26 Jun 2014
 

Abstract

A reversed-phase high-performance liquid chromatography method using a diode array detector at 260 nm was developed and validated for the determination of arbutin, niacinamide, and adenosine in cosmetics. The analytes were extracted using methanol and deionized water. The separations were performed on a C18 column with gradient elution. At the optimized conditions, the limits of detection for arbutin, niacinamide, and adenosine were 0.75, 0.12, and 0.01 µg/mL, respectively. The average recoveries were between 98.1% and 107.5%. The method was used to analyze thirty cosmetics.

Notes

a y: peak area of analyte; x: concentration of analyte (µg/mL).

*Concentration of adenosine.

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