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Abstract
The goal of this study was to develop and validate a high-performance liquid chromatography method for the quantification of two essential oil constituents (myristicin and linalool) from extracts of the three aromatic plants exposed to low-intensity microwave radiation. The analysis was performed by high-performance liquid chromatography using a mobile phase consisting of ultrapure water and acetonitrile. The separations were carried out in the following conditions: isocratic conditions for 30 minutes with 100% acetonitrile and then linear gradient conditions from 100% acetonitrile to 25% acetonitrile for 15 minutes. The developed method was validated in terms of linearity, limits of detection, limits of quantification, precision, accuracy, and robustness. The results show that plants react differently under microwave stress and change their essential oil content as a response.