Determination of Phenolic Compounds and the Antioxidant Capacity of Ximenia parviflora Benth. var. parviflora (Olacaceae) Fruit by High-Performance Liquid Chromatography with Diode Array Detection

ABSTRACT

The total phenolic and flavonoid content, phenolic composition, and in vitro antioxidant capacity of ethanolic extracts of Ximenia parviflora Benth. var. parviflora fruits collected at Zinaparo, Michoacan (in central Mexico) were determined. Fruit extracts present a high scavenging activity of 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] radicals (71.49 ± 0.11% and 85.00 ± 1.29% inhibition, respectively). The four phenolic compounds identified in fruit extracts by high-performance liquid chromatography with diode array detection were gallic acid, chlorogenic acid, caffeic acid, and quercetin. X. parviflora fruits may be used as a starting material for the extraction of high value antioxidant phenolic compounds with potential applications in the pharmaceutical and dietary supplement industries.

KEYWORDS:

• Antioxidant capacity
• high-performance liquid chromatography with diode array detection (HPLC-DAD)
• phenolic compounds
• scavenging activity
• Ximenia parviflora

Additional information

Funding

Authors JRMM, MVS and EVL thank the CONACYT for the financial support, the Instituto Politecnico Nacional and the CIIDIR IPN Unidad Michoacan for allowing us the use of its laboratories.