Determination of Tetracyclines in Milk with a Molecularly Imprinted Polymer-Based Microtiter Chemiluminescence Sensor

Abstract

A molecularly imprinted polymer (MIP) capable of recognizing five tetracyclines using minocycline as the template was synthesized for the first time. The MIP was employed as the recognition reagent to prepare a chemiluminescence sensor on a conventional microtiter plate. The light signal was initiated using the highly efficient bis(2,4,6-trichlorophenyl) oxalate-hydrogen peroxide-imidazole chemiluminescence system. After optimization of several appropriate factors, the sensor was employed to determine five tetracyclines in milk. The developed assay contained only one sample-loading step, so each measurement was completed within 12 min. The limits of detection for these analytes were in the range from 0.5 to 2.0 pg/mL, while the recoveries from the fortified milk samples were between 78.1 and 105%. In addition, the sensor was shown to be reusable for up to four measurements. Hence, this sensor has been demonstrated to be a simple, rapid, sensitive, and durable tool for the determination of tetracyclines in animal-derived food.

Keywords:

• Microtiter chemiluminescence sensor
• milk
• minocycline
• molecularly imprinted polymer
• tetracyclines

Additional information

Funding

This study was financed by the Hebei Key Research and Development Project (18226628D), and the Training Program of Innovation and Entrepreneurship for Undergraduate, Hebei Agricultural University (2017127, 201810086010).