Determination of β-Agonists in Porcine Urine by Molecularly Imprinted Polymer Based Chemiluminescence

Abstract

Isoprenaline was used as the template to synthesize a molecularly imprinted polymer that was able to simultaneously recognize ten β-agonists. The simulation showed that the three-dimensional conformation of the template was the main factor responsible for the polymer’s recognition. The polymer particles were coated in the wells of a conventional microplate as the recognition reagent to prepare a chemiluminescence sensor. The light signal was initiated with a highly effective bis(2,4,6-trichlorophenyl) oxalate-H₂O₂-imidazole system. The assay contained only one sample-loading step followed by immediate data acquisition, so one measurement was complete within 12 min. The sensor was used to determine the ten analytes in porcine urine. The results showed that the senor achieved ultrahigh sensitivity, with limits of detection from 2.0 to 7.0 pg/mL, high recoveries from 78.6% to 99.4%, and satisfactory recycle performance up to seven times. This is the first study reporting a molecularly imprinted polymer based microtiter chemiluminescence sensor for the determination of β-agonists.

Keywords:

• β-agonists
• chemiluminescence
• isoprenaline
• molecularly imprinted polymer
• porcine urine

Funding

This study was financed by the Hebei Key Research and Development Project (18226628D) and the Training Program of Innovation and Entrepreneurship for Undergraduates at Hebei Agricultural University (2017127, 201810086010).