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Electrochemistry

Electrochemical amplification for Hg(II) quantification by anchoring an enzymatically extended aptamer

, , , , , , , & show all
Pages 2883-2895 | Received 25 Feb 2019, Accepted 29 May 2019, Published online: 12 Jun 2019
 

Abstract

A novel indirect electrochemical approach is reported to improve the sensitivity of Hg2+ quantification in environmental water samples. This strategy is based on the Hg2+ induced selective interaction of thymine mismatched pairs in a thymine-rich aptamer DNA to improve the specificity, while template-independent terminal deoxynucleotidyl transferase (TdT) extended the 3′-OH aptamer termini with repeated bases and increased the sensitivity. The introduction of the target Hg2+ blocked aptamer extension. Taking advantage of an electrochemical platform, this aptamer-TdT based amplification sensor enabled the specific quantification of Hg2+ ion over a wide linear range from 2 pM to 20 nM with a low limit of detection equal to 0.1 pM. The proposed strategy is promising for the detection of other metal ions.

Disclosure statement

The authors declare no competing financial interests.

Additional information

Funding

This research was supported by the Foundation of Yunnan Province [2017ZE027, 2016CP021], the Basic Research Foundation of Yunnan Tobacco Industry [2018JC04], and the Foundation of Key Laboratory of Tobacco Chemistry of Yunnan Province [KCFZ-2017-1096].

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