Determination of the Volatiles in Rosa chinensis Cultivars by Comprehensive Two-Dimensional Gas Chromatography (GC × GC) and Quadrupole Time-of-Flight (QTOF) Mass Spectrometry (MS)

Abstract

The Rosa chinensis cultivars Rosa Lvye, Rosa Knock out, Rosa Parade and Slater’scrimson China were analyzed for the volatile chemical composition in their flower petals. Headspace solid phase microextraction (HS-SPME) was applied to extract volatile flavors, and comprehensive two-dimensional gas chromatography (GC × GC) and quadrupole time-of-flight (QTOF) mass spectrometry (MS) were used for chemical profiling, including quantitative and qualitative analysis of the Rosa cultivars. A total of 97 volatile compounds were identified and 38 to 67 compounds were identified in each cultivar. The total concentrations of the extracted volatile compounds in the four cultivars were Rosa Knock out (750.21 ± 45.06 μg/g) > Rosa Parade (714.02 ± 6.94 μg/g) > Rosa Lvye (558.11 ± 9.08 μg/g) > Slater’s crimson China (436.86 ± 3.21 μg/g). This study provides a specific chromatographic fingerprint for the Rosa chinensis cultivars, which can be applied in differentiation of the fragrance and aroma constituents.

Keywords:

• Rosa chinensis
• volatiles
• headspace solid phase microextraction (HS-SPME)
• comprehensive two-dimensional gas chromatography (GC) quadrupole time-of-flight (QTOF) mass spectrometry (MS)
• GC-MS

Additional information

Funding

This study was supported by the Fundamental and Public Project of Guizhou Academy of Agricultural Sciences ([2020]15) and the National Agriculture Product Risk Assessment Project (GJFP2018005).