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Biosensors

Dual Aptamer-Copper (II) Phosphate Nanocomposite-Based Point-of-Care Biosensor for the Determination of Escherichia coli O157:H7 through Pressure Monitoring with a Hand-Held Barometer

, , , , , & show all
Pages 1603-1615 | Received 26 Nov 2019, Accepted 25 Aug 2020, Published online: 11 Sep 2020
 

Abstract

Here, two types of aptamer (specific to E. coli O157:H7 pathogenic bacteria) Cu3(PO4)2 nanocomposites loaded with Fe3O4 nanoparticles (aptamer@Fe3O4, magnetic separation) and platinum nanoparticles (PtNPs) (aptamer@PtNP) were prepared in house via enzymatic hydrolysis of H2O2 to O2 through a one-step method. In the proposed point-of-care biosensor, these nanocomposites were used as capture and signal probes for the determination of pathogenic E. coli O157:H7 bacteria via hand-held barometers detecting gas pressure signals in a sealed bottle. The pressure signal was linear when the concentration of E. coli O157:H7 was from 103 to 108 CFU/mL. With the easily prepared dual aptamer-Cu3(PO4)2 nanocomposites and the hand-held barometer signal readout, the proposed point-of-care biosensor was shown to be a potential tool for the determination of pathogenic bacteria in underdeveloped areas.

Disclosure statement

The authors declare that there are no conflicts of interest.

Additional information

Funding

This work was financially supported by the National Key Research and Development Program of China (Grant Number 2016YFD0501000).

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