339
Views
7
CrossRef citations to date
0
Altmetric
Liquid Chromatography

High-Performance Liquid Chromatography with Fluorescence Detection for the Determination of Capsaicin and Dihydrocapsaicin in Fat-Burning Dietary Supplements

, , &
Pages 2097-2112 | Received 12 Sep 2020, Accepted 17 Oct 2020, Published online: 08 Jan 2021
 

Abstract

Recent studies show that the weight loss process may be supported by compounds isolated from plants. Capsaicinoids, primarily capsaicin and dihydrocapsaicin, are the active alkaloids from chili peppers, which have been classified into this group of compounds and are widely used in dietary supplements. Due to the mild legal regulations being in force in the European Union regarding dietary supplements, concentrations of capsaicin and dihydrocapsaicin have been tested in six local products. The optimization of the capsaicin and dihydrocapsaicin extraction process was performed using central composite design. The developed procedure involved 60 minutes of ultrasound-assisted extraction with 65% methanol. High-performance liquid chromatography (HPLC) with fluorescence detection was used to analyze the extracts from the supplements. However, capsaicinoids were determined in considerably smaller concentrations than those declared by the producers.

Additional information

Funding

This work was supported by the Polish Ministry of Science and Higher Education grant number 0911/SBAD/0396.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.