Isolation of Volatiles in Honeysuckle by Multiple Approaches with Determination by Gas Chromatography–Mass Spectrometry (GC–MS) and Headspace Solid-Phase Microextraction-Gas Chromatography–Mass Spectrometry (HS-SPME/GC–MS)

Abstract

The volatile components of honeysuckle were determined using hydro-distillation (HD), water extraction coupling rectification (WER), and water extraction coupling rectification with solvent extraction (WER-SE). Gas chromatography–mass spectrometry (GC–MS) and headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME/GC–MS) identified 127 compounds. There were 27 common compounds extracted by these methods. A heatmap of the 27 common compounds indicates that WER-SE and WER were complementary approaches. In addition, 59 compounds were detected for the first time in honeysuckle. The results of this study provide a reference for the determination of volatile components in plants.

Keywords:

• Headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME/GC-MS)
• honeysuckle
• volatile components
• water extraction coupling rectification
• water extraction coupling rectification and solvent extraction

Disclosure statement

The authors declare that they have no conflicts of interest.

Additional information

Funding

This work received financial support from the Shanghai Science and Technology Innovation Action Project (No. 19391902400).