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Spectrophotometry

Robust Tag-Free Aptasensor for the Visual Determination of Lead Ion Based upon the Peroxidase-Catalytic Activity of Gold Nanoparticles

ORCID Icon, ORCID Icon, ORCID Icon, , & ORCID Icon
Pages 1407-1416 | Received 27 Jan 2023, Accepted 16 Aug 2023, Published online: 01 Sep 2023
 

Abstract

Here, a simple colorimetric strategy was developed to monitor lead ion (Pb2+) based upon the catalytic function of gold nanoparticles (AuNPs) regulated by the aptamer strands. The peroxidase-like activity of AuNPs was prevented by the aptamers through masking their surface. Thus, the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) did not proceed. By adding Pb2+, the aptamer-Pb2+ complex was formed that released the aptamer from the surface of AuNPs. Hence, free AuNPs initiated the oxidation of TMB that changed the solution color from colorless to blue. A linear concentration range was achieved from 0.2 to 200 nM with a detection limit (LOD) of 0.056 nM for Pb2+ detection. The aptasensor successfully monitored Pb2+ in drinking water, milk, and human serum. With the properties of sensitivity, simplicity, and naked-eye target monitoring, the developed aptamer-included biosensor (aptasensor) is suitable for food safety, health care application, and environmental monitoring.

Disclosure statement

The authors declare that they have no competing interests.

Additional information

Funding

This study was financed by Iran National Science Foundation (INSF) as a research project (Project 99012771).

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