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ANALYTICAL BIOCHEMISTRY AND CLINICAL ANALYSIS

Fluorescence Lifetimes of Fluorescent Substances Formed in Peroxidized Microsomes of Rat Liver

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Pages 57-65 | Received 01 Nov 1989, Accepted 23 Nov 1989, Published online: 23 Oct 2006
 

Abstract

Detection of the time-resolved decay of the fluorescence emitted from lipid peroxidized membranes succeeded using peroxidized microsomes of rat liver. Analysis of the time resolved decay showed that three components were most adequate to fit the observed decay. This suggests the heterogeneity of the fluorescent substances related to the species of fluorophores, their forms and/or their environment. Measurements of the fluorescence lifetimes of the fluorescent substances will be of great use to further characterize the fluorescent substances.

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