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Abstract
A novel, sensitive enzyme coupling procedure is described for the quantitative determination of both purified bovine milk lipoprotein lipase (LPL) and human post-heparin plasma lipolytic activity (PHLA). The primary assay is carried out with the aid of a triolein emulsion optimized as regards NaCl, bile salt and C II apolipoprotein concentration. The secondary reaction consists of the photometric measurement of a colored adduct formed following enzymatic oxidation of non esterified fatty acids. The use of radiolabels is thus eliminated. The precision of the assay was comparable to other competent methods (CVs obtained are between 2.1 and 9.5). The system described is sensitive enough for detecting post-heparin plasma lipolytic activity in both healthy humans and cancer patients.