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Abstract
We describe a new colorimetric method for the zinc assay in serum. The method uses the chromogen 4-(2-pyridylazo) resorcinol and, since it does not require any preconcentration step or specialized instrumentation and personnel, it is suitable for routine work in small laboratories. The correlation with the atomic obsorption spectroscopy method applied to wet-ashed samples is high (r2=0.9826). The mean within-assay CV is 6.7% and the mean between assay CV is 7%. The method was tested on a sample of 1253 individuals randomly chosen. The mean zinc concentration was statistically different between males and famales.