Determination of some antioxidants by derivative spectrophotometric method and high-performance liquid chromatography

Abstract

The UV-derivative spectrophotometric method was used for assaying butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and propyl gallate (PG) in pure state and in laboratory mixtures. Ir; this paper the results are presented using zero-crdssing technique. In this way, thc interference of two components mixed together has been eliminated. That is the greatest advantage of derivative spectroscopy compared with the classical UV-spectrophotometric method. After analysing the criteria f o r the application of this method, the amounts of these substances were determined. Simul taneously, a highperformance liquid chromatography was used.

Key Words:

• Butylated hydroxyanisole
• butylated hydroxytoluene
• propyl gallate
• third and fourth derivative spectrophotometry
• two component mixtures
• high-performance liquid chromatography