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Abstract
An enzymatic-high performance liquid chromatography method with electrochemical detection was developed for the quantitation of p-chloro-m-xylenol conjugated metabolites in blood plasma and urine. The method is based on enzymatic hydrolysis of PCMX conjugates and extraction with benzene. The HPLC system consisted of a C18 reverse-phase column and a 65% methanol:35% ammonium carbonate 0.05% solution as a mobile phase. An electrochemical detector/glassy carbon electrode set at a potential of +0.9V versus Ag/AgCl/3M NaCl was used to monitor the drug. The recovery of PCMX after enzymatic hydrolysis from blood plasma samples and urine samples is approximately 98%.