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Abstract
A simple, accurate and reproducible liquid chromatographic procedure is developed for separating three sulfonamides. The procedure has been developed to separate and quantitate sulfaguanidine, sulfadiazine and succinyl sulfathiazole, in powdered form to be used as oral suspension, using sulfanilic acid as internal standard. A C18 reverse-phase column (25 cm × 4.6 mm i.d.; 10 μm particles) was used. The mobile phase was 20% methanol in 0.05 M ammonium acetate delivered at a flow rate of 1 mL/min. The UV detection was set at 270 nm with a band-width of 10 nm. The separation of the three sulfas was completed in about five minutes. The method met the USP requirments for system suitability and linearity (r values of 0.999 or better). The extraction of the sulfa drugs from the powder is performed by a very simple procedure. This procedure is useful for monitoring the production process of the pharmaceutical preparation containing the three sulfonamides.