Preparation and Characterization of IgG-Coated Membranes for Possible Use as Solid Phases in Enzyme Immunosensors

Abstract

The preparation and characterization of rabbit and bovine IgG-coated membranes is discussed. The performance of these membranes in a competitive binding assay for rabbit IgG and in a sandwich assay for rabbit anti-bovine IgG were used to judge the potential usefulness of these membranes as solid phases for enzyme immunosensors. In particular, these membranes were intended for use in enzyme immunosensors based on the ammonia gas-sensing electrode which employed urease labeled immunoreagents.

Key Words:

• IgG
• Enzyme
• Immunosensor