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Analytical Letters Volume 25, 1992 - Issue 2
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Chemometiucs

The Application of a Three Factor Central Composite Experimental Design to The Paper Chromatographic Separation of Several Amino Acids

Frederick H. Walters Department of Chemistry, University of Southwestern Louisiana, Lafayette, LA, 70504-4370
Pages 353-362 | Received 13 Feb 1991, Accepted 30 Sep 1991, Published online: 23 Sep 2006
 
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Abstract

A three factor central composite design was used to study the effect of methanol, propanol and amyl alcohol on the paper chromatographic separation of arginine, histidine, leucine, threonine and tryptophan. Statistical analysis of the Rf data yields second order equations which describe the response surface of Rf as a function of the composition of the 3 alcohols. This data is useful in understanding the relative influence of each alcohol and in predicting Rf values for different solvent combinations.

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Related Research Data

The use of a Mixture Design to Analyze the Effects of Different Alcohols on the Paper Chromatographic Separation of Amino Acids and Amino Acid Hydroxamates
Source: Informa UK Limited
The use of a 25 factorial design to study the effect of various alcohols on the paper chromatographic separation of selected amino acids
Source: Informa UK Limited
5 – AMINES, AMINO ACIDS, PEPTIDES, AND PROTEINS
Source: Elsevier
Chapter V – AMINO ACIDS, AMINES AND PROTEINS
Source: Elsevier
Thin-layer chromatography of amino acids☆
Source: Elsevier BV
A review of response surface methodology from a biometric viewpoint.
Source: JSTOR

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