Abstract
A rapid fluorimetric method is reported for the determination of thiamine based upon its interaction with Co(II) ion in the presence of base and a nonionic Triton X-100 micellar medium. The effect of different experimental variables upon the fluorescence intensity were assessed. The detection limit for thiamine is 5.0 × 10−8M (λex =375nm, λem =433nm). The method was successfully applied for determination of thiamine in variety of samples with good accuracy being achieved.
Key Words: