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Spectrometry

Fluorimetric Determination of Thiamine with Cobalt(II)

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Pages 2283-2289 | Received 23 Mar 1993, Accepted 21 May 1993, Published online: 23 Sep 2006
 

Abstract

A rapid fluorimetric method is reported for the determination of thiamine based upon its interaction with Co(II) ion in the presence of base and a nonionic Triton X-100 micellar medium. The effect of different experimental variables upon the fluorescence intensity were assessed. The detection limit for thiamine is 5.0 × 10−8M (λex =375nm, λem =433nm). The method was successfully applied for determination of thiamine in variety of samples with good accuracy being achieved.

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