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Analytical Letters Volume 26, 1993 - Issue 11
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Spectrometry

Determination of Benzo(a)pyrene in Water by Synchronous Fluorimetry Following Preconcentration on Sephadex Gels

L. F. Capitán-Vallvey Department of Analytical Chemistry, University of Granada, 18071, Granada, Spain
,
M. del Olmo Iruela Department of Analytical Chemistry, University of Granada, 18071, Granada, Spain
,
R. Avidad Castañeda Department of Analytical Chemistry, University of Granada, 18071, Granada, Spain
&
J. L. Vilchez Quero Department of Analytical Chemistry, University of Granada, 18071, Granada, Spain
Pages 2443-2454 | Received 02 Mar 1993, Accepted 21 May 1993, Published online: 23 Sep 2006
 
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Abstract

Benzo(a)pyrene (BaP) which presents native fluorescence in solution has been determined in waters by synchronous solid-phase spectrofluorimetry. The relative fluorescence intensity of BaP fixed in Sephadex G-25 gel is measured after being packed in an 1-mm silica cell. Spectral characteristics of BaP-gel system are described. The applicable concentration range was 0.52–3.45 μg.L−1 with a relative standard deviation of 1.2% and detection limit of 0.07 μg.L−1. The method was applied to the analysis of waters with different provenances.

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