Statistical Mixture Design Applied to RP-HPTLC Chromatographic Separations of Amino Acid Hydroxamates

Abstract

Mixture Design and a Chromatographic Response Function were used to study the effect of different organic solvents as modifiers on the Reversed Phase (C-18) High Performance Thin Layer Chromatographic separation of three amino acid hydroxamates (Arginine hydroxamate, Threonine hydroxamate, and Histidine hydroxamate). Both 3 solvent (methanol, acetonitrile and tetrahydrofuran) and 4 solvent (methanol, acetonitrite, tetrahydrofuran and acetone) systems were studied.

Key Words:

• Mixture design
• amino acid hydroxamates
• reversed phase high performance thin layer chromatography