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Analytical Letters Volume 27, 1994 - Issue 15
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Original Articles

A Fast Made Disposable Glucose Biosensor Incorporating the Oxidase and a Ferrocene in an Alginate Gel

Alexander D. Ryabov Division of Chemistry, G. V. Plekhanov Russian Economic Academy, Stremyanny per. 28, 113054, Moscow, Russia
,
Marina Ivanova Department of Chemistry, Moscow State University, 119899, Moscow, Russia
,
Boris B. Gnedenko Department of Chemistry, Moscow State University, 119899, Moscow, Russia
&
R. Valunas§ Department of Chemistry, Moscow State University, 119899, Moscow, Russia
Pages 2813-2821 | Received 15 Jun 1994, Accepted 31 Jul 1994, Published online: 22 Aug 2006
 
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Abstract

A simple procedure is described for co-immobilization of glucose oxidase and dimethylaminomethylferrocene in a sodium alginate gel on the surface of pyrolytic graphite electrode. The film is electrochemically active and the peak current is a function of D-glucose concentration with the apparent Michaelis-Menten constant Km = (1.2±0.3) × 10−2 M. The optimal concentration range for biosensoric applications in 0.001–0.010 M.

§On leave from the Institute of Applied Enzymology FERMENTAS, Vilnius, Lithuanian Republic.

Notes

§On leave from the Institute of Applied Enzymology FERMENTAS, Vilnius, Lithuanian Republic.

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