Advanced search
Publication Cover
Analytical Letters Volume 27, 1994 - Issue 7
Submit an article Journal homepage
13
Views
10
CrossRef citations to date
0
Altmetric
Original Articles

Non-enzymatic Deproteinization of Immobilized DNA Suitable for Pulsed Field Gel Electrophoresis.

D. Higginson Centro Nacional de Investigaciones Cientificas, Departamento de Biologia Molecular, Avenida 25 # 15202 y Calle 158, Playa, Apartado 6880, Habana, CUBA
,
G. Perez Centro Nacional de Investigaciones Cientificas, Departamento de Biologia Molecular, Avenida 25 # 15202 y Calle 158, Playa, Apartado 6880, Habana, CUBA
,
L. Lopez-Canovas Centro Nacional de Investigaciones Cientificas, Departamento de Biologia Molecular, Avenida 25 # 15202 y Calle 158, Playa, Apartado 6880, Habana, CUBA
&
A. M. Riveron Centro Nacional de Investigaciones Cientificas, Departamento de Biologia Molecular, Avenida 25 # 15202 y Calle 158, Playa, Apartado 6880, Habana, CUBA
Pages 1255-1264 | Received 24 Sep 1993, Accepted 04 Feb 1994, Published online: 22 Aug 2006
 
Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days

Abstract

Agarose immobilized DNA samples suitable for pulsed field gel electrophoresis (PFGE) were prepared by lysis of immobilized cells followed by disruption of DNA-protein complexes with denaturant and detergent solutions. The electrophoretic pattern of samples deproteinized by this method were identical to samples treated with the fungal protease Proteinase K.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.