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Analytical Letters Volume 27, 1994 - Issue 8
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Original Articles

TTF-Modified Biosensors for Hydrogen Peroxide

Laura Bifulco Hydrocontrol, Centro di Ricerca e Formazione per il Controllo dei Sistemi Idrici, Poggio dei Pini, 09012, Capoterra, CA, Italy
,
Cosimo Cammaroto Hydrocontrol, Centro di Ricerca e Formazione per il Controllo dei Sistemi Idrici, Poggio dei Pini, 09012, Capoterra, CA, Italy
,
Jeffrey D. Newman Cranfield Biotechnology Centre, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK
&
Anthony P.F. Turner Cranfield Biotechnology Centre, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK
Pages 1443-1452 | Received 13 Dec 1993, Accepted 01 Mar 1994, Published online: 22 Aug 2006
 
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Abstract

Amperometric biosensors for hydrogen peroxide were produced, based on TTF-modified graphite disc electrodes, incorporating immobilised peroxidase. At the optimum operating potential (−200 mV vs. calomel electrode), a linear response to hydrogen peroxide was obtained in the range 0–2 mM (Km value = 1.98 mM). Low sensitivity to pH fluctuations and good stability at room temperature were exhibited.

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