Abstract
A model of an oximeter-based two-substrate enzyme electrode is presented. The simplifications of the general model lead to a solution to differential equations describing the influence of the rate of the enzyme reaction in combination with the diffusion phenomena on measurable oxygen flux. Both transient phase and steady state conditions for experimental data can be considered within the frames of the model and provide possibilities for the calibration of the biosensor. The optimal parameters for the biosensor calibration can be used in practical design of enzyme electrodes.