Detection of the red tide-causing plankton Alexandrium affine by a piezoelectric immunosensor using a novel method of immobilizing antibodies

Keijiro Nakanishi Research Center for Advanced Science and Technology, University of Tokyo, 4–6–1 Komaba, Meguro-ku, Tokyo, 153, Japan

Adachi Masao Faculty of Agriculture, Kochi University, Nangoku city, Kochi Pref. 783, Japan

Yoshihiko Sako Faculty of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto, 606, Japan

Yuzaburo Ishida Faculty of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto, 606, Japan

Hitoshi Muguruma Research Center for Advanced Science and Technology, University of Tokyo, 4–6–1 Komaba, Meguro-ku, Tokyo, 153, Japan

Isao Karube Research Center for Advanced Science and Technology, University of Tokyo, 4–6–1 Komaba, Meguro-ku, Tokyo, 153, Japan

Abstract

In this paper, the determination of Alexandrium affine in sea water was performed using piezoelectric immunosensors. In addition, we report a novel method of immobilizing antibodies on the quartz crystal immunosensors using plasma-polymerized ethylenediamine film. The films formed on the quartz crystals are extremely thin, homogeneous and incorporate amino groups. Sensors produced using this method show higher sensitivity than sensors made using conventional immobilization methods for example polyethylenimine or γ-aminopropyltrimethoxysilane.

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Detection of the red tide-causing plankton Alexandrium affine by a piezoelectric immunosensor using a novel method of immobilizing antibodies

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Detection of the red tide-causing plankton Alexandrium affine by a piezoelectric immunosensor using a novel method of immobilizing antibodies

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