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Abstract
An enzyme sensor for the detection of sulfonylurea herbicides inhibiting acetolactate synthase II (ALS) was developed using an oxygen electrode. ALS, which has an oxygen consumption side reaction, was entrapped in the matrix of PVA-SbQ polymer, and the enzyme membrane was attached to the electrode. The inhibition of side oxygen reaction of ALS II is measured as decreased consumption of O2 monitored by an oxygen electrode. Preliminary results show that 10−6 M herbicide concentration can be determined by this method.