Chemiluminescence Sensor with Mn(III)-Tetrakis (4-sulfonatophenyl)-porphyrin Immobilized on Dioctadecyldimethylammonium Chloride Bilayer Membranes Incorporated Into PVC Film

Abstract

An indicator phase for a flow-through chemiluminescence (CL) sensor composed of ordered surfactant assemblies, a polymer and a catalyst was evaluated by measuring adrenaline. The method is based on use of Mn (III)-porphyrin immobilized on a bilayer membrane contained in a blended film, prepared by incorporating dioctadecyl-dimethylammonium chloride into polyvinyl chloride. The sensor consisted of a Pyrex glass tube (30 mm × 5 mm i.d.) packed with silica glass wool, on which the indicator phase was coated, and a photomultiplier tube. The blend film functioned as a favorable reaction medium for the adrenaline CL, and further enhanced CL was observed with the immobilized catalyst. This indicator phase permitted adrenaline to be detected down to 3 × 10⁻⁶ M with a 20 μl injection into a 0.3 M NaOH carrier solution. The relative standard deviation (n = 10) was 1.0% for 5 × 10⁻⁵ M adrenaline. For 80 successive injections of 5 × 10⁻⁵ M adrenaline, the variation of the CL signal was within the relative standard deviation. Almost the same sensitivity and precision were observed with the indicator phase stored in water for at least 3 days. The sensor was successfully applied to determine adrenaline in drug samples.

Key Words:

• chemiluminescence sensor
• Mn (III)-porphyrin complex
• surfactant bilayer membrane
• bilayer/PVC blend film
• adrenaline