A Glucose Selective Electrode Based on Calix[6]Arene and Application in a Human Serum Assay

ABSTRACT

Plasticized poly(vinyl chloride) (PVC) membranes incorporating dealkylcalix[6]arene as a sensory material were developed for the determination of glucose after its in situ derivatization with a lipophilic Girard's P′ reagent. In a TRIS-HCl buffer at pH 9.0, the electrode exhibits a near Nernstian response in the range 1.0 × 10⁻⁴ - 0.01 mol l⁻¹ glucose with a slope of 45.5 mV per concentration decade. The electrode has a fast response time and a long working lifetime. The selectivity and sensitivity of this electrode method allow its application to the assay of glucose concentration in human blood samples.

Keywords:

• Glucose determination
• Girard's type reagents
• ion-selective electrode
• calixarene
• host-guest interaction