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An extractive-spectrophotometric study based on the reaction of furosemide with 1,2-naphthoquinone-4-sulphonate (NQS) is described. Optimal conditions are: NaH2PO4-Na2HPO4 pH 7.5, NQS 7.7×10−3M, heating 30 min at 70° C and isoamyl alcohol as extractant. The method is used to determine furosemide in pharmaceutical samples. The interference caused by lactose can be eliminated using pH 4.5. The method can be also used to determine lactose at pHs 4.5 and 7.5.
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