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Analytical Letters Volume 31, 1998 - Issue 4
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Original Articles

A Fluorescence-Based Sensor for Ammonium and Nitrate

Satoshi Sasaki Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153
,
Yoshihiro Ando Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153
,
Makiko Dejima Faculty of Science, Department of Chemical and Biological Sciences, Japan Women's University, 2-8-1 Mejirodai, Bunyo-ku, Toyo, 112
,
Yoshiko Arikawa Faculty of Science, Department of Chemical and Biological Sciences, Japan Women's University, 2-8-1 Mejirodai, Bunyo-ku, Toyo, 112
&
Isao Karube Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153
Pages 555-567 | Received 14 Nov 1997, Accepted 20 Nov 1997, Published online: 22 Aug 2006
 
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Abstract

Measurement of nitrite and nitrate in rain samples was performed using a fluorescence based sensor. Nitrite and nitrate were reduced to ammonia using Devarda's alloy, and the gas was then passed through a membrane and reacted with o-phthalaldehyde. A linear relationship between the nitrate concentration and the luminescence intensity was observed over the concentration range of 1 – 5 mg/1, with a relative standard deviation (RSD) of 2.3% at a nitrate concentration of 1 mg/1 (n = 5). The system did not show any response toward sulfate or chloride. Results obtained from the measurement of river water samples using the sensor showed good agreement with those obtained using a conventional method.

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