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Abstract
Monitoring of cyclosporine A in 40 whole blood samples from renal transplantation patients by high performance liquid chromatography with aryl oxalate-sulforhodamine 101 chemiluminescence detection is described. These data were compared with fluorescence polarized immunoassay (Cyclosporine-SP DynapackTM using monoclonal antibody for whole blood), with good results (r = 0.88, y = 0.80 + 5.07). These values are the same as reported by high performance liquid chromatography with photometric detection. By using a solid phase extraction cartridge, cyclosporine A was analyzed within 30 min. The analysis time could be shortened to one tenth that previously reported. The proposed method has the same sensitivity as fluorescence polarized immunoassay.