Determination of Pyrogallol Using a Novel Chemiluminescence Reaction

Abstract

The chemiluminescence reaction of lucigenin-hydroxylamine hydrochloride-NaOH is enhanced by pyrogallol. This provides a new chemiluminescence method for the determination of micro amounts of pyrogallol. The detection limit of pyrogallol is 4.5 × 10⁻⁸ g/ml, and the linear dynamic range is 5.6 × 10⁻⁸ g/ml to 5.6 × 10⁻⁶ g/ml. The relative standard deviation of ten replicate measurements is 2.3% for 10⁻⁷ g/ml pyrogallol. The interference study shows that this method has good selectivity. The percent recovery is 98.5–105%.

Key Words:

• chemiluminescence
• lucigenin
• pyrogallol
• hydroxylamine hydrochloride