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Abstract
A bulk acoustic wave (BAW) impedance sensor has been applied to rapid determination of in vitro concentrations of cefotaxime sodium. The method based on the growth frequency curve of Proteus Morganii, inhibited by cefotaxime sodium, is continuously monitored by using a BAW impedance sensor. The growth kinetic parameter, e.g. asymptote or maximum frequency shift (AF, Hz), estimated by fitting a modified Gompertz model to the growth frequency curve, is proportional to the logarithm of antibiotic concentration. The proposed method exhibits a favourable response to 0.25 – 20.0 μg mL−1 cefotaxime sodium with a detection limit of 0.03 μg mL−1, and the recoveries range from 97.3 to 107.5% (n=9). The results are in good agreement with those obtained by the conventional disk diffusion test (DDT) method. The effects of temperature and pH on the determination are discussed and in vitro susceptibility to cefotaxime of the genus Proteus has been investigated in detail.
On leave from Department of Mathematics, Physics and Mechanics, Changsha Railway University.
Notes
On leave from Department of Mathematics, Physics and Mechanics, Changsha Railway University.