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Abstract
A method has been developed for the qualitative and quantitative determination of enzymatic lipolysis products in the presence of high triglyceride concentrations, using aminopropyl bonded silica cartridges and capillary gas chromatography. Two reactions are catalyzed in the presence of the Rhizopus javanicus lipase: hydrolysis of triglycerides to diglycerides, and diglycerides to monoglycerides. Lipids were extracted from the hydrolyzed olive oil emulsion and separated into fractions with aminopropyl bonded silica cartridges by means of sequential elution. The elution procedure was well characterized and optimized to obtain a maximum recovery and purity for each lipid fraction. Further analysis was done with capillary gas chromatography.
The method is suitable to study the properties of lipases in vitro in the presence of high triglyceride concentrations and different amounts of bile salts. The expected inhibition of the fungal lipase by higher concentrations of bile salts, under physiological conditions, was limited.