Application of Thiocyanate Complex of Titanium (IV) to the Extractive Spectrophotometric Determination of Amitriptyline Hydrochloride

Abstract

Amitriptyline hydrochloride reacts in acidic medium with the thiocyanate complex of titanium (IV) forming a yellow ion- association compound, insoluble in water but fairly soluble in a mixture of chloroform and butyl alcohol (4:1). The formation and extraction conditions of the compound are described. The compound was characterized by UV, VIS, IR and ¹H NMR spectrometry. The colored compound exhibits a maximum absorption at 360 nm. Under the optimum experimental conditions, amitriptyline hydrochloride was determined in the 3-60 μg/cm³ range with a relative standard deviation less than 0.5% (n=8). The proposed method was verified against the pharmacopoeial method and applied to the determination of amitriptyline hydrochloride in pharmaceuticals.

Key Words:

• amitriptyline hydrochloride
• extractive spectrophotometry
• Ti(IV)