Spectrofluorimetric Determination of Iproniazid and Isoniazid in a FIA System Provided with a Solid-Phase Reactor

Abstract

A FIA assembly is proposed for the fluorimetric determination of iproniazid and isoniazid. The oxidation of both drugs is carried out by H₂O₂. The excess of reagent is destroyed and removed from the flow-injection manifold by means of a metallic copper reactor which acts as catalyst in the decomposition of H₂O₂ and a home made debubbler. The calibration graphs were linear up to 14 μg ml⁻¹ and 10 μg ml⁻¹ (n=6) for iproniazid and isoniazid, respectively, with limits of detection of 0.008 μg ml⁻¹ and 0.005 μg ml⁻¹. The sample throughput was 24 h⁻¹ for both drugs. The influence of foreign compounds was studied and the procedure was applied to determination of iproniazid and isoniazid in pharmaceutical formulations.

Key Words:

• Iproniazid
• isoniazid
• FIA
• spectrofluorimetry