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ABSTRACT
A novel silica-based amide-polymer-bonded packing to be used for protein separation is described. A macroporous silica support was bonded with diethoxymethylvinyl silane, and then copolymerized with methylacrylamide and divinylbenzene to produce a tailored stationary phase. This packing has high resolution and stability in a large pH range; it was characterized through the separation of a standard protein mixture containing bean trypsinogen inhibitor, glucose oxidase, ribonuclease and pepsin. Such manipulation of the proteins elution profile is achievable in less than 12 minutes. The effect of pH on the protein separation is discussed, as is the effect of ammonium sulfate concentration. It is suggested that the macroporous silica-based amide-polymer-bonded packing is a better alternative for the biopolymer separation.