The Use of Derivative Spectrophotometry for the Determination of Acyclovir and Diloxanide Furoate in Presence of Impurity or Degradation Product

Abstract

Second (D₂) and third (D₃) derivatives spectrophotometry have been developed for the determination of acyclovir in the presence of guanine (its main impurity) and diloxanide furoate in the presence of diloxanide (its degradation product). The methods depend upon measuring D₂ and D₃ peak amplitudes at 269 and 262 nm for acyclovir and at 260 and 270 nm for diloxanide furoate in 0.1 N hydrochloric acid.

Accuracy of analysis with the proposed derivative spectrophotometric procedure is significantly greater than the classical methods.

Key Words:

• acyclovir
• guanine
• impurity
• diloxanide furoate
• degradation product
• second and third derivative spectrophotometry.