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Original Articles

A Study on the Reaction of Protein with Arsenazo III by Rayleigh Light Scattering Technique

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Pages 1689-1701 | Received 04 Mar 1998, Accepted 20 Apr 1998, Published online: 22 Aug 2006
 

Abstract

A new rayleigh light scattering (RLS) assay of protein was conducted in this paper. The weak RLS of arsenazo III can be enhanced greatly by the addition of proteins. Based on this, the reaction of arsenazo III and proteins was studied. A new quantitative determination method for proteins has been developed. This method is very sensitive (0.085(021.25 μg/mL for BSA), rapid (<1min), simple (one step) and free of interference from most diverse substances.

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