Analysis of Reverse Phase Chromatographic Separated Bacillus subtilis Membrane Proteins

Abstract

B. subtilis membrane proteins were separated by reverse phase chromatography using a simple solvent system of acetonitrile/water. The isolated proteins were further characterized by a ‘MS-FIT’ peptide mass fingerprinting program. The experimental scheme for separation and identification of membrane proteins could also be modified to detect protein-protein interactions. The method has been proven useful for rapid isolation, purification, and characterization of hydrophobic membrane proteins.

Key Words:

• Hydrophobic membrane proteins
• reverse phase chromatography
• simple solvent system
• protein-protein interactions
• matrix
• MALDI-TOF analysis
• peptide mass fingerprinting
• Bacillus subtilis