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Abstract
A reversed-phase high performance liquid chromatography method (HPLC) has been developed for the identification and the quantitation of tamoxifen, desmethyltamoxifen and didesmethyltamoxifen in plasma using a solid phase extraction. A Waters Symmetry C8 3.5μm column (4.6 × 100 mm) was used, with a mobile phase of 50% acetonitrile in 100 mM ammonium acetate buffer at pH 5.2. Analysis of the plasma samples was carried out at concentrations ranging from 30 to 500 ng/ml. The solutes were detected at 238 nm with a detection limit for tamoxifen and its demethylated metabolites as low as 5 ng/ml. Such procedures can be routinely applied to the determination of tamoxifen and its two demethylated metabolites in plasma from patients undergoing tamoxifen therapy.