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Original Articles

Determination of Sterols and their Esters in Fats by Way of Transesterification in Different Solvents

Pages 811-825 | Published online: 18 Feb 2008
 

ABSTRACT

The interference of triglycerides in the analysis of sterols and their esters in fats can be eliminated by transesterification of the sample dissolved in n-hexane with a solution of sodium hydroxide 2 mol/L. Since the analytes are distributed equally in n-hexane and in methanol, better results can be obtained by accurate measurements of the volumes, and processing the standards as the samples. Triglycerides and steryl esters are transesterified quantitatively by a solution of sodium hydroxide 2 mol/L using diethyl ether as solvent. At 40°C, ten minutes are sufficient to complete the reaction. The sterols are determined in fats using a mass spectrometer detector, at concentrations as low as 5 mg/Kg. Using FID, it was possible to analyze sterols present in concentrations higher than 100 mg/Kg.

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