Micellar Electrokinetic Capillary Chromatography as an Alternative Method for the Determination of Estrogens

ABSTRACT

A micellar electrokinetic chromatographic method which permits the separation of very similar compounds such as ethinylestradiol (ETE), gestodene (GTD), (-)norgestrel (LEV) and β-estradiol valerate (EST) from synthetic mixtures is described. The study was carried out at 25°C and 20 kV, using a 15 mM borate buffer (pH 9.2), 15 mM sodium dodecylsulfate (SDS) and 26% acetonitrile-water as background electrolyte. The analysis takes about 12 minutes.

Keywords::

• Ethinylestradiol
• Gestodene
• (-)norgestrel
• β-estradiol valerate
• Micellar electrokinetic chromatography (MEKC)
• Estrogens