Cobalt(II)Hexacyanoferrate Film Modified Glassy Carbon Electrode for Construction of a Glucose Biosensor

ABSTRACT

An amperometric glucose biosensor was constructed based on a glassy carbon electrode modified with a Cobalt(II)hexacyanoferrate film which catalyzes electroreduction of hydrogen peroxide. Gelatin was used as immobilization matrix. Interference could be effectively eliminated by the combination of low detection potential with a Nafion coating. A low applied potential can avoid oxidation of interferences such as ascorbic acid, uric acid, p-acetyl-aminophenol, etc.. Nafion coating prevents interferences from access to the electrode surface by electrostatic repulsion. A wide linear range of detection was obtained. Analytical performance parameters are given and kinetic analysis discussed.

Keywords::

• Cobalt(II)hexacyanoferrate
• Glucose oxidase
• Biosensor
• Modified electrode
• Amperometry